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pcdna5 construct  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcdna5 construct
    Pcdna5 Construct, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna5+construct/Tetracycline/pm41145429-229-13-28
    Average 99 stars, based on 1 article reviews
    pcdna5 construct - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: The human cytoplasmic dynein interactome reveals novel activators of motility
    Article Snippet: .. Stable cell lines were generated by transfection with Lipofectamine 2000 (Thermo Fisher Scientific) and a combination of the appropriate pcDNA5 construct and pOG44, which expresses Flipase. ..

    Article Title: Structural basis of drug recognition by human MATE1 transporter
    Article Snippet: Human MATE1 (Uniprot ID: Q96FL8 ) sequence was codon-optimized for expression in human cells (GeneArt, ThermoFisher) and purchased from GenScript. .. The synthetic gene was cloned into pcDNA5 vector containing 3C-cleavable N-terminal Flag-eYFP tag. pcDNA5 construct was used for tetracycline-inducible stable cell line generation (Flp-InTM T-RExTM 293 Cell Line, Thermo Fisher Scientific), following the manufacturer’s guidelines. .. Cells were grown and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 100 μg/mL streptomycin, 100 units/mL penicillin (Thermo Fisher Scientific) at 37 °C with 5% CO 2 under humidified conditions.

    Article Title: Structural basis of drug recognition by human MATE1 transporter.
    Article Snippet: Construct design and protein expression Human MATE1 (Uniprot ID: Q96FL8) sequence was codon-optimized for expression in human cells (GeneArt, ThermoFisher) and purchased from GenScript. .. The synthetic gene was cloned into pcDNA5 vector containing 3C-cleavable N-terminal Flag-eYFP tag. pcDNA5 construct was used for tetracycline-inducible stable cell line generation (Flp-InTM T-RExTM 293 Cell Line, Thermo Fisher Scientific), following the manufacturer’s guidelines. .. Cells were grown and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 100μg/mL streptomycin, 100 units/mL penicillin (Thermo Fisher Scientific) at 37 °C with 5% CO2 under humidified conditions.

    Generated:

    Article Title: The human cytoplasmic dynein interactome reveals novel activators of motility
    Article Snippet: .. Stable cell lines were generated by transfection with Lipofectamine 2000 (Thermo Fisher Scientific) and a combination of the appropriate pcDNA5 construct and pOG44, which expresses Flipase. ..

    Transfection:

    Article Title: The human cytoplasmic dynein interactome reveals novel activators of motility
    Article Snippet: .. Stable cell lines were generated by transfection with Lipofectamine 2000 (Thermo Fisher Scientific) and a combination of the appropriate pcDNA5 construct and pOG44, which expresses Flipase. ..

    Construct:

    Article Title: The human cytoplasmic dynein interactome reveals novel activators of motility
    Article Snippet: .. Stable cell lines were generated by transfection with Lipofectamine 2000 (Thermo Fisher Scientific) and a combination of the appropriate pcDNA5 construct and pOG44, which expresses Flipase. ..

    Article Title: Structural basis of drug recognition by human MATE1 transporter
    Article Snippet: Human MATE1 (Uniprot ID: Q96FL8 ) sequence was codon-optimized for expression in human cells (GeneArt, ThermoFisher) and purchased from GenScript. .. The synthetic gene was cloned into pcDNA5 vector containing 3C-cleavable N-terminal Flag-eYFP tag. pcDNA5 construct was used for tetracycline-inducible stable cell line generation (Flp-InTM T-RExTM 293 Cell Line, Thermo Fisher Scientific), following the manufacturer’s guidelines. .. Cells were grown and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 100 μg/mL streptomycin, 100 units/mL penicillin (Thermo Fisher Scientific) at 37 °C with 5% CO 2 under humidified conditions.

    Article Title: Structural basis of drug recognition by human MATE1 transporter.
    Article Snippet: Construct design and protein expression Human MATE1 (Uniprot ID: Q96FL8) sequence was codon-optimized for expression in human cells (GeneArt, ThermoFisher) and purchased from GenScript. .. The synthetic gene was cloned into pcDNA5 vector containing 3C-cleavable N-terminal Flag-eYFP tag. pcDNA5 construct was used for tetracycline-inducible stable cell line generation (Flp-InTM T-RExTM 293 Cell Line, Thermo Fisher Scientific), following the manufacturer’s guidelines. .. Cells were grown and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 100μg/mL streptomycin, 100 units/mL penicillin (Thermo Fisher Scientific) at 37 °C with 5% CO2 under humidified conditions.

    Clone Assay:

    Article Title: Structural basis of drug recognition by human MATE1 transporter
    Article Snippet: Human MATE1 (Uniprot ID: Q96FL8 ) sequence was codon-optimized for expression in human cells (GeneArt, ThermoFisher) and purchased from GenScript. .. The synthetic gene was cloned into pcDNA5 vector containing 3C-cleavable N-terminal Flag-eYFP tag. pcDNA5 construct was used for tetracycline-inducible stable cell line generation (Flp-InTM T-RExTM 293 Cell Line, Thermo Fisher Scientific), following the manufacturer’s guidelines. .. Cells were grown and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 100 μg/mL streptomycin, 100 units/mL penicillin (Thermo Fisher Scientific) at 37 °C with 5% CO 2 under humidified conditions.

    Article Title: Structural basis of drug recognition by human MATE1 transporter.
    Article Snippet: Construct design and protein expression Human MATE1 (Uniprot ID: Q96FL8) sequence was codon-optimized for expression in human cells (GeneArt, ThermoFisher) and purchased from GenScript. .. The synthetic gene was cloned into pcDNA5 vector containing 3C-cleavable N-terminal Flag-eYFP tag. pcDNA5 construct was used for tetracycline-inducible stable cell line generation (Flp-InTM T-RExTM 293 Cell Line, Thermo Fisher Scientific), following the manufacturer’s guidelines. .. Cells were grown and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 100μg/mL streptomycin, 100 units/mL penicillin (Thermo Fisher Scientific) at 37 °C with 5% CO2 under humidified conditions.

    Plasmid Preparation:

    Article Title: Structural basis of drug recognition by human MATE1 transporter
    Article Snippet: Human MATE1 (Uniprot ID: Q96FL8 ) sequence was codon-optimized for expression in human cells (GeneArt, ThermoFisher) and purchased from GenScript. .. The synthetic gene was cloned into pcDNA5 vector containing 3C-cleavable N-terminal Flag-eYFP tag. pcDNA5 construct was used for tetracycline-inducible stable cell line generation (Flp-InTM T-RExTM 293 Cell Line, Thermo Fisher Scientific), following the manufacturer’s guidelines. .. Cells were grown and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 100 μg/mL streptomycin, 100 units/mL penicillin (Thermo Fisher Scientific) at 37 °C with 5% CO 2 under humidified conditions.

    Article Title: Structural basis of drug recognition by human MATE1 transporter.
    Article Snippet: Construct design and protein expression Human MATE1 (Uniprot ID: Q96FL8) sequence was codon-optimized for expression in human cells (GeneArt, ThermoFisher) and purchased from GenScript. .. The synthetic gene was cloned into pcDNA5 vector containing 3C-cleavable N-terminal Flag-eYFP tag. pcDNA5 construct was used for tetracycline-inducible stable cell line generation (Flp-InTM T-RExTM 293 Cell Line, Thermo Fisher Scientific), following the manufacturer’s guidelines. .. Cells were grown and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 100μg/mL streptomycin, 100 units/mL penicillin (Thermo Fisher Scientific) at 37 °C with 5% CO2 under humidified conditions.



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